The enzyme-linked immunosorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell.

Jul 3, 2025 · The ELISPOT (enzyme-linked immunospot) assay is a powerful immunological technique used to detect and quantify secreted proteins, such as cytokines, at the single-cell level.

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

ELISPOT is the most sensitive technique available for the detection of immune cells which secrete specific signature proteins such as antibodies or cytokines. It is the state-of-the-art method for the …

EliSpot (Enzyme linked immuno spot) is a technology, to evaluate a functional immnue answer of T or B cells to different antigens. On this site we try to answer all open questions you may have regarding …

Nov 10, 2022 · ELISpot is an immunoassay used to quantify analyte-secreting cells. Cytokines, immunoglobulins, or other target proteins secreted by cells are captured by specific antibodies …

May 23, 2022 · ELISpot has evolved from a research tool to an important clinical assay. Recent studies of cancer vaccines tested in a variety of malignancies have suggested that ELIspot may be a useful …

Key issues. Enzyme-linked immunosorbent spot (ELISpot) is a highly flexible assay and can be adapted to multiple readout formats. ELISpot assays are quantitative and measure key cellular functions of …

ELISPOT Wash Buffer: 1 L of 1X PBS 0.5 mL Tween-20 Alternately, powdered ELISA/ELISPOT wash buffer can be purchased (Cat. No. 00-0400) and reconstituted with 1 L DI H2O

The ELISpot assay is a hybrid assay that combines the features of a mixed lymphocyte reaction (MLR) assay and an enzyme-linked immunosorbent assay (ELISA). In it's main principle, the ELISpot …